TotalLab TL120 v2008.01
TotalLab TL120 v2008.01
TotalLab TL120 v2008.01 英文正式版(生物醫學類圖像分析軟體)
一個圖像分析軟體，對DNA、蛋白凝膠電泳圖像、arrays, dot blots與colonies等圖像可以
The TL120 version in the TotalLab range is an advanced image analysis
solution which offers an extensive range of features for the in-depth
analysis of 1D electrophoresis gels and performing band pattern matching
Offers a flexible and intuitive workflow
The steps you follow to analyse your 1D gel are logically ordered,
making the analysis process straightforward but still offering complete
flexibility. The TL120 software also provides a facility to setup
analysis protocols which can be applied to your experiments allowing
batch processing of images under the same conditions, ensuring a uniform
Contains highly-sophisticated algorithms to cope with the most demanding
To accurately analyse your 1D gel, the lanes you loaded on your gel and
the resultant bands must be accurately detected by the TL120 software.
There is a choice of algorithms for fully automatic and highly accurate
lane creation which have been optimised to cope with images that are
badly distorted. Band material is precisely detected which is vital for
Enables Rf calibration which drives highly accurate molecular weight
Rf lines are used in 1D analysis to account for any distortions and
variations in your gel that may have occurred during one of the
experimental stages. TL120 contains an Rf calibration facility that
allows you to position lines and adjust Rf values. When Rf lines are
positioned consistently across the gels in an experiment, inter-gel
comparisons can be made.
This calibration can be subsequently used to drive accurate molecular
weight calibration and eventual lane relationship studies.
Provides an extensive range of tools for accurate quantitation and
The TL120 software has a wide range of tools for quantitation and
normalisation to cater for all your experimental requirements.
First you must subtract the background material which is automatically
and precisely calculated by TL120. There is a choice of quantity
calibration options including the facility to create multiple curves per
gel, useful for a dilution series, and a range of tools for
normalisation of band material. Additional features which facilitate
highly accurate quantitation include the option to perform profile
deconvolution on merged / poorly resolved bands, and tools to account
for band "grimacing".
Performs precise band pattern matching
The highly flexible matching facility matches bands across your 1D gel
within tolerances you define using Rf values, molecular weight
calibration or lane position. You can nominate a lane on your gel to
match to, or match to an artificial reference lane, generated by the
software, which contains all the bands present within the gel. The
results can be viewed as a dendrogram which allows the identification of
band patterns on a single gel.
Contains advanced visualisation and reporting tools
The TL120 software provides a range of views of your gel data to assist
with the analysis. These include a dendrogram view, a choice of table
options to view band matching results and a histogram view to illustrate
lane data. The 1D image window and lane profile views can also be
completely customised to suit your analysis
Detailed reports are generated with a single click and can be tailored
to present results for an individual lane or complete gel. Reports to
show quantity calibration and molecular weight data are also
And when your analysis is complete?
If you wish to perform subsequent mass spec analysis and use a robot
cutter, TL120 can coordinate and control precise picking for a wide
range of instruments.
Finally, your analysed experiment can be sent to the TL120 DM database
component for further data mining and in-depth lane relationship